CHAPTER 1
An Illustrated Glossary of Histological and Pathological Terms
3
Cytoplasm Features
Basophilia versus acidophilia (eosinophilia)
: In a
hematoxylin and eosin (H&E)–stained section,
basophilic components stain blue-purple; aci-
dophilic components stain pink-red. The
arrow
indicates the basal part of the cytoplasm of a cell
of a salivary gland duct. The acidophilic (
pink
)
staining results from a concentration of mito-
chondria in this part of the cell. The
arrowhead
indicates the cytoplasm of a plasma cell. The
basophilic (
bluish
) staining results from a con-
centration of rough endoplasmic reticulum.
Granules
: These vesicles stain because they retained at least
some of their contents during tissue preparation. The
arrow
indicates small acidophilic granules F lling the cytoplasm of
an eosinophil. The
arrowhead
indicates large acidophilic
secretory granules in the apical cytoplasm of a Paneth cell.
Note that the basal cytoplasm surrounding the nucleus of
the Paneth cell stains basophilic, indicating large amounts of
rough endoplasmic reticulum in this region of the cell.
Vacuolated cytoplasm
: This type of cytoplasm contains what appear to
be empty holes. Usually, these represent either lipid droplets or vesicles
whose contents were washed out during processing of the tissue. What
appear to be empty spaces in the cells from the adrenal cortex shown
here were actually occupied, in the living state, by droplets of lipid
(cholesterol) that were extracted during tissue preparation.
Abundant versus scant
: This pair of terms describes a substantial
amount (volume) of cytoplasm (
cell 1
) or a slight amount of cyto-
plasm (
cell 2
) surrounding the nucleus. These are inexact terms but
are sometimes useful in describing a cell.
20
μ
m
20
μ
m
20
μ
m
20
μ
m
20
μ
m
20
μ
m
1
2
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